Automated and Network Structure Preserving Segmentation of Optical Coherence Tomography Angiograms
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Optical coherence tomography angiography (OCTA) is a novel non-invasive imaging modality for the visualisation of microvasculature in vivo. OCTA has encountered broad adoption in retinal research. OCTA potential in the assessment of pathological conditions and the reproducibility of studies relies on the quality of the image analysis. However, automated segmentation of parafoveal OCTA images is still an open problem in the field. In this study, we generate the first open dataset of retinal parafoveal OCTA images with associated ground truth manual segmentations. Furthermore, we establish a standard for OCTA image segmentation by surveying a broad range of state-of-the-art vessel enhancement and binarisation procedures. We provide the most comprehensive comparison of these methods under a unified framework to date. Our results show that, for the set of images considered, the U-Net machine learning (ML) architecture achieves the best performance with a Dice similarity coefficient of 0.89. For applications where manually segmented data is not available to retrain this ML approach, our findings suggest that optimal oriented flux is the best handcrafted filter enhancement method for OCTA images from those considered. Furthermore, we report on the importance of preserving network connectivity in the segmentation to enable vascular network phenotyping. We introduce a new metric for network connectivity evaluations in segmented angiograms and report an accuracy of up to 0.94 in preserving the morphological structure of the network in our segmentations. Finally, we release our data and source code to support standardisation efforts in OCTA image segmentation.
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